Composite

Part:BBa_K3258028:Design

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-21)


MDV + CAM Flipped


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 180
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
    Illegal XhoI site found at 168
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 180
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 180
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part designed to allow the demonstration of Qbeta in replicating target gene constructs, which is only possible with Qbeta to allow the production of sense CAM resistance gene.


Source

Synthetic construct derived from CAM resistance gene from E. coli and MDV regions.

References